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the recrystallizing in the fragmental synthesis of the Lys 26 side chain in the preparation method is performed with one or a mixture of two or more of Et 2 O, CH 2 Cl 2 , CHCl 3 , MeOH, CH 3 CN, THF, EtOAc, EtOH, Toluene, Hexane, or Acetone, preferably a mixed solvent of MeOH and CH 2 Cl 2

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For the PAG analysis, counts were performed across 0.21 mm postrior to bregma 2.488 mm (two sections were selected, 30 m in thickness, one in every seven)