Since D2HG and L2HG are indistinguishable in standard LC-MS, to determine which 2HG enantiomer(s) is affected, we adapted a chiral N-(p-toluenesulfonyl)-L-phenylalanyl chloride (TSPC) derivation followed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis 24,40
Funding This work was supported by the National Science and Technology Major Project (2022ZD0117700), the National Natural Science Foundation of China (32270759) and the Research Foundation for Postdoctoral (AUGA5710026124)
doi: 10.18502/ijrm.v20i11.12365 7 DomecqJPPrutskyGMullanRJSundareshVWangATErwinPJet al
The whole process takes about 30 to 45 minutes, and many patients report feeling refreshed and energized afterward
Your body produces glutathione on its own, but GSH levels can drop due to stress, poor diet, lack of sleep, or exposure to toxins and pollutants