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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

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Current research models of SLU-PP-332 typically use subcutaneous injections, with efforts underway to develop an oral tablet for future human trials

From a male fertility perspective, carnitine is of interest because the male reproductive system naturally contains high levels of it, and sperm motility requires energy available in the right place