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Energy minimization was conducted with the steepest descent method (maximum force tolerance of 1000 kJmolnm, step size 0.01 nm), followed by 100 ps NVT equilibration (0310.15 K) and 100 ps NPT equilibration (310.15 K, 1 bar) using the Berendsen barostat

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These outcomes were characterized by greater callus development and increased mineralization within the affected area, indicating enhanced bone continuity and strength (21)