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The suspended liver microsomes were placed on ice in prechilled microcentrifuge tubes, allowing for the addition of 640 ul of 0.1 M Tris buffer and 10 ul of 500 uM test compound to the sample

Possible side effects include: Mild pain, redness, or swelling at the injection site Headache or dizziness (usually transient) Nausea (uncommon) Hypokalaemia (low potassium) during initial treatment, particularly in severely deficient patientsthis is rare but may require monitoring [1] [5] When to seek medical advice: Serious adverse reactions are extremely rare

Not included: Syringes or bacteriostatic water

The compound to be soaked was maintained during the cryo-protection process