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avoid repeated freeze-thaw cycles IGF-1 LR3 activates the IGF-1 receptor (IGF-1R), a receptor tyrosine kinase, initiating autophosphorylation of intracellular tyrosine residues and downstream activation of the PI3K/Akt/mTOR and Ras/MAPK/ERK signaling cascades, which are studied in the context of cell proliferation, protein synthesis regulation, and apoptosis suppression across a broad range of cell lines

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A 2025 comprehensive review in Medical Science Monitor covering studies from 20162025 confirmed that GHK-Cu-based formulations enhance fibroblast migration, ECM remodelling, collagen and elastin synthesis, and wound closure across multiple model types