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For expression and purification of RAG GTPases, HEK293F GnTI- cells were transfected with a total of 1 mg plasmid DNA (550 g for RAGA and 450 g for RAGC) and 4 mg polyethylenimine (PEI) (Sigma-Aldrich) per l at a density of 1.8 10 6 cells per ml

Lifelong B12 shots may be needed for pernicious anemia or irreversible malabsorption

Although this is an attractive approach that spurred great interest, caution should be exercised as standard dosing may be a challenge depending on individual inhalation technique

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