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Ben: It's awful

The hypoxic tumor microenvironment is considered one of the biggest obstacles for the development of anticancer drugs

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However, inhibition of USP7 is sufficient to alter the ubiquitylation of a subset of age-affected proteins involved in proteostasis, cytoskeletal dynamics, trafficking, and synaptic transmission

Briefly, dissected gonads were fixed in 3% paraformaldehyde/0.1M K 2 HPO 4 (pH 7.2) solution for 10-20 min, and then post-fixed with cold 100% methanol for 5 min (Alternatively, fixed worms can be stored in cold methanol at -20C for a few days)