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Nrf2 knockdown prevents NFT-dependent increase in cellular glutathione levels To examine the correlation between Nrf2 activation and the increase in glutathione levels after NFT treatment, Nrf2 protein levels were experimentally lowered by siRNA-mediated knockdown, which was confirmed by Western blotting (Fig

To evaluate whether group differences in histological outcomes remained significant when accounting for subject- or animal-level variability, we reanalyzed all relevant datasets using linear mixed-effects models