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It might be worth getting someone with more expertise in biomedical research to review the studies in this literature review

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Samples were then extracted and cleaned according to AOAC Method 2007.01 for pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulphate (REFERENCE: available online at Ragworm Samples: Fifty milligram ragworm samples were weighed into 2 mL tubes and spiked with internal standard solution (10 L of a solution containing 500 ng/mL nicotine-D4 and cotinine-D3) together with 300 L water (2% ammonium hydroxide) and 400 L acetonitrile

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Reply Robustojoe I think this problem is a function of temperature