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Participants randomized to provide saliva return their samples to the study center, where they are given another sample kit to complete the day before the next study visit

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Measurement of intracellular ROS Intracellular ROS levels were measured in intact 16-HBE cells by the 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) assay using acetyl-ester (Molecular Probes, Carlsbad, CA, USA) according to the manufacturers instructions

Research has suggested that the vapor contains far fewer harmful compounds than cigarette smoke, but probably more than are present in the vapor from e-cigarettes