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For control group, 180 l fresh culture medium and 20 l of sterile water were added

For immunofluorescence, cryosections were dried for 2 h at RT, fixed in pure acetone for 10 min and blocked with PBS 10% FCS for 30 min at RT

Researchers used NMDA receptor antagonists (APV), GABA receptor antagonists (BIC), and AMPA/KA receptor antagonists (DNQX) in order to pharmacologically isolate and observe evoked currents

Wang YH, Yao N, Wei KK, Jiang L, Hanif S, Wang ZX, Pei CX

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