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Materials and methods Tobacco samples and culture conditions The first-cured tobacco samples KRK26 were collected from Yanshan County, Wenshan Prefecture, Yunnan Province, China in 2020 and used as the test material
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PBMC stimulation To stimulate PBMCs, they were cultured in RPMI 1640 containing 10% FBS and 10 g/mL gentamycin
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