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However, when KA is oriented at the entrance of the active site, it restricts substrate entrance and product efflux, consequently, TyrBm cannot reach its maximum velocity (Table 1)

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After supernatant alkalization, samples were reacted with an equal volume of 2,4-dinitrofluorobenzene (FDNB) solution [1.5% (v/v) in ethanol] for 3 h at room temperature in the dark