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The pellet was resuspended in 1 mL PBS, loaded onto a 7 mL layer of 11% w/v Accudenz (Accurate Chemical) and centrifuged for 30 min at 7000 g at 4 C without brake (Sorvall RC-5B Superspeed Centrifuge with HB-4 swing-out rotor)

Schoenfeld, Howard (1950) The Danbury Story. In Prison Etiquette: The Convicts Compendium of Useful Information by The Inmates, p