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SLC7A11 knockdown using shRNA Short hairpin mediated knockdown of SLC7A11 in FLO-1 and OE33 cells were performed by cloning SLC7A11 -specific shRNA into a lentiviral tetracycline-inducible expression vector containing the optimized miR-E backbone (LT3GECIR) 40

The peptide activates melanocortin-1 receptors and upregulates tyrosinase enzyme production, but tyrosinase needs UV-induced oxidative stress to catalyze the chemical reactions that polymerize melanin

A nonpaired t test was used to compare between groups