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The libraries were further quantified on a Qubit 2.0 fluorometer using Qubit dsDNA HS kit (Life Technologies, USA) and by quantitative PCR (qPCR) using KAPA SYBR FAST qPCR Master mix and Illumina standards and primer premix (KAPA Biosystems, Wilmington, MA, USA), following the Illumina-suggested protocol

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Macrophage phagocytosis of apoptotic neutrophils is compromised by matrix proteins modified by cigarette smoke and lipid peroxidation products