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The inherent anti-inflammatory and antioxidant effects of melatonin also contribute to the overall health of the male reproductive system

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25(OH)VD, insulin, glucose, GSH, protein carbonyl, and Malondialdehyde (MDA) assays Plasma levels of 25(OH)vitamin D were determined using an ELISA kit (Calbiotech, Spring Valley, CA), levels of insulin were determined using ELISA kits from ALPCO Diagnostics (Salem, NH)