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Colony formation assay In brief, 100 cancer cells per well were plated in six-well plates in triplicates and cultured with nicotine (1 M) or CM from human primary neutrophils treated with nicotine or HDN/LDN CM from mouse primary neutrophils for 7 days as indicated before staining viable colonies with 0.5% crystal violet solution (Sigma-Aldrich)

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If enacted, the new laws will remove all logos, trademarks, colours, designs and graphics except for the make or name of the product which will be in a uniform typeface on a plain background

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