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Because anaerobic glycolysis of glucose to pyruvate by cells produces protons and lactate [51, 52], we analyzed the glycolytic activity of endometrial stem cells exposed or not to cigarette smoke by measuring real-time extracellular acidification rates (ECARs) after treating cells with 2-deoxyglucose (2-DG) to inhibit glycolytic activity, and thus, obtain information on basal ECAR