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To perform Na + dependence assays, carnitine doses were applied to oocytes expressing hOCTN2, and related mutants, in the presence of Cl - buffers of differing Na + concentration (10100 mM NaCl, 100 mM - [NaCl] CsCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , 5 mM HEPES, buffered to pH 7.4 with Tris base)

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