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The acidic pH from ascorbic acid disrupts the copper-peptide bond

Freeze-thaw cycling degrades peptide backbones and can additionally disrupt the copper coordination sphere

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The typical outcome measure used by researchers is the Sciatic Function Index that assesses hind limb motor function based on toe to toe and toe to heel distance

Several factors have been shown to reduce the permeability of the blood-brain barrier (BBB) (da Fonseca et al., 2014) (Figure 1)