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However, the effect wears off over time, leaving the person tired and craving another cigarette to reenergize them

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Scott, an author of the study examining smokings effect on the brain, said in a news release

Immunblots were performed from cell lysate to analyze the expression of different cell cycle and apoptotic markers, such as, p53, p21, Bax, Bcl-2 and from serum to measure the level of different pro-inflammatory cytokines, such as, IL-6, TNF-, iNOS, Cox-2

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