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After the proteins were transferred to the membrane, it was blocked with 510 ml of Li-Cor blocking buffer (LI-COR Biosciences, Lincoln, NE) for 1 hour to prevent the nonspecific binding of antibodies to its surface
The effector protein PthA is the most important factor in the production of canker symptoms in host plants 8
Non-nicotine medications: Prescription medications such as bupropion and varenicline target nicotine receptors in the brain to reduce cravings and the rewarding effects of tobacco use
Retrieved 1 March 2019
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