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It dissolves well in DMSO at 1mg/ml However, adding water may cause cloudiness In that case, applying ultrasonic treatment for 2 seconds usually clears the solution Consequently , proper handling ensures accurate experimental results and consistent performance

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Furthermore, trypsin mRNA expression as a marker for acinar cell contamination was assessed using PCR (primers: Table 1, PCR conditions: Tm=60 C, cycles 40)

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