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Just like the use of a handkerchief as a surrogate wind sock is not permitted

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For millions, especially among lower socioeconomic groups, smokeless tobacco is not merely a habitit is an addiction that begins in adolescence and continues for decades

2.2 Determination of endogenous hormone in the tobacco plant and root Tobacco plant endogenous hormones, including IAA, JA, GA, and ABA, were measured using enzyme-linked immunoassay (ELISA) ( 2.3 Protein extraction, digestion, and desalting The protein extraction, digestion, and desalting steps followed the methods as described in previous publications ( 2.4 iTRAQ labeling and fractionation The peptides were dissolved in a 0.5-ml TEAB solution and then labeled according to the instructions provided by the iTRAQ-8 standard kit from SCIEX