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The relative expression of target genes was calculated using the following formula: 2 ^(CT) (Livak and Schmittgen 2001)

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Examining the adaptive processes that are established to respond to the initial CS burden may help to understand what pathways become defective or are compromised and may contribute to the pathogenesis of COPD

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The implications of centrally produced, neuromodulatory GLP-1 in the context of GLP-1RAs are uncertain, as the degree to which signals from pre-proglucagon neurons/endogenous GLP-1 system and GLP-1RA activity converge on shared downstream targets is unclear 6 and may in fact occur independently 7