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The second hexa-histadine tag of the protein was not utilized in these experiments, but is relevant and must be noted because Ni-NTA was used to purify the GST-GFP for several of the experiments being discussed
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the secondary antibody used for the visualization of COX2 was IRDye 800CW Donkey anti-Rabbit (925-32213, LI-COR) at a 1:30,000 dilution
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