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Sorted single-cell suspensions were resuspended at an estimated final concentration of 1000 cells/l and loaded on a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM)

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Glutaredoxins are therefore key enzymes for redox catalysis and physiological processes such as DNA synthesis, signal transduction, and organ development 2,21,34,38,40,47,48

By using lysine derivatives with different side-chain protecting groups (such as Fmoc-Lys(Alloc)-OH or Fmoc-Lys(Dde)-OH), chemists can achieve regioselective side-chain modifications to construct head-to-tail cyclic peptides or side-chain-to-side-chain cyclic peptides